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Culturing marine benthic dinoflagellates (F2 and G media)
Provided by:
Shauna Murray
School of Biological Sciences
University of Sydney
Sydney NSW, AUSTRALIA
email:smurray@bio.usyd.edu.au
Culturing marine benthic dinoflagellates
Cultures were made of some species, and maintained at 20 ° C and a 12 hour light cycle, using f/2 medium made up in sterile seawater at approximately 35 ‰ salinity.
Contents of F2 and G media used for culturing marine benthic dinoflagellates.
Compound |
F/2 |
G medium |
NaNO 3 |
0.075 g/L |
|
KNO 3 |
|
0.1 g/L |
NaH 2PO 4.2H 2O |
0.005 g/L |
|
K 2HPO 4 |
|
0.0348 g/L |
Na 2SiO 3.9H 2O 3.9H 2O |
0.015 g/L |
|
Na 2EDTA |
|
0.03 g/L |
FeCl 3.6H 2O |
|
0.00145 g/L |
FeC 6H 5O 7.3H 2O
|
0.0045 g/L
|
|
| C 6H 8O 7.H 2O | 0.0045 g/L | |
H 3BO 3 |
|
0.034 g/L |
CuSO 4.5H 2O |
1 x 10 -5 g/L |
|
ZnSO 4.7H 2O |
2.2 x 10 -5 g/L |
|
ZnCl 2 |
|
3 x 10 -4 g/L |
CoCl 2.6H 2O |
1 x 10 -5 g/L |
1.3 x 10 -4 g/L |
MnCl 2.4H 2O |
1.8 x 10 -4 g/L |
4.3 x 10 -3 g/L |
| Na 2MoO 4.2H 2O | 6 x 10 -6 g/L | |
Thiamine hydrochloride |
1 x 10 -4 g/L |
1 x 10 -3 g/L |
| Vitamin B12 (Cyanocobalamin) | 0.5 mg/L | 1 x 10 -6 g/L |
Biotin |
|
2 x 10 -6 g/L |
Soil Extract |
|
5 x 10 -3 g/L |
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